cd86 fitc  (Miltenyi Biotec)

Journal: Bioactive Materials

Article Title: A self-locking conductive cardiac patch for immediate electrical integration with infarcted rat myocardium

doi: 10.1016/j.bioactmat.2025.10.045

Figure Lengend Snippet: The effects of patches on angiogenesis and inflammation in infarcted tissues 4 weeks post-MI. a) Immunofluorescence staining of α-smooth muscle actin (α-SMA, a vascular protein marker, green) and von Willebrand factor (vWF, an endothelial marker, red) of the infarct region in the MI, BMN-P, BMN-CP and CBMN-CP groups. b, c) Quantitative analysis of α-SMA (b) and vWF (c) in different groups based on fluorescent staining images (n = 4). d) Representative immunofluorescence staining of the infarct region in different groups for CD86 (green) and CD206 (red). e, f) Fluorescence intensity statistics of CD86 (e) and CD206 (f) in different groups based on fluorescent staining images (n = 4). Nuclei are stained blue with DAPI. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The tissue sections were then incubated with the following primary antibodies: rabbit Connexin 43 primary antibody (BOSTER, BA1727, 1:200, China), mouse α-actinin primary antibody (Abcam, AB9465, 1:200, UK), mouse α-SMA primary antibody (Wuhan Sanying, 67735-1-IG, 1:400, China), rabbit vWF primary antibody (Wuhan Sanying, 27186-1-AP, 1:300, China), mouse CD86 primary antibody (BOSTER, BA4121, 1:100, China) and rabbit CD206 primary antibody (Wuhan Sanying, 18704-1-AP, 1:400, China).

Techniques: Immunofluorescence, Staining, Marker, Fluorescence

Journal: Materials Today Bio

Article Title: NO-driven self-propelled nanomotors with deep biofilm penetration for synergistic therapy of peri-implantitis

doi: 10.1016/j.mtbio.2025.102658

Figure Lengend Snippet: Anti-inflammatory effects of IHPS nanomotors on macrophage polarization. (A) Schematic diagram of the immunomodulatory mechanism. (B) Relative mRNA expression levels of M1 macrophage markers (IL-6, TNF-α, iNOS) after various treatments. (C) Relative mRNA expression levels of M2 macrophage markers (IL-4, CD206, ARG-1). (D) Representative immunofluorescence staining of iNOS and CD206; nuclei were stained with DAPI (Scale bar: 20 μm). (E, F) Quantitative analysis of the fluorescence intensity of iNOS and CD206. (G) Flow cytometry analysis of the surface expression of M1 marker CD86 and M2 marker CD206. Data are presented as mean ± SD (n = 3) ( ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001).

Article Snippet: Antibodies specific for iNOS, CD86, CD206, OCN, OPN, PKG1, sGC, Runx2, and β-actin were obtained from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence, Flow Cytometry, Marker